Fig. 2 | Scientific Reports

Fig. 2

From: Fluorescence Guided Raman Spectroscopy enables the training of robust support vector machines for the detection of tumour marker proteins

Fig. 2

Expression of Cx43-mTagBFP2 in HEK293 cells. (a) Predicted protein models for mTagBFP2, Cx43, and Cx43-mTagBFP2 (light blue: β-pleated sheets, red: α-helices). (b, c) Confocal fluorescence images of HEK293 expressing Cx43-mTagBFP2 show the colocalization of Cx43-mTagBFP2 and the anti-Cx43 antibody. Square image: view of the XY-plane, right image: orthogonal view of the YZ-plane along the vertical blue or white line, bottom image: orthogonal view of the XZ-plane along the horizontal blue or white line. (d) Widefield epifluorescence image showing the colocalization of Cx43-mTagBFP2 (blue) and anti-Cx43 antibody (red) at the characteristic membranous locations. (e) Cropped western immunoblot with an anti-Cx43 antibody (bottom) and an anti-vinculin antibody (loading control, top) (1: blot ladder with molecular weight indicated on the left, 2: HEK293 cells expressing Cx43-mTagBFP2, 3: HEK293 wildtype). The fusion protein is observed at around 70 kDa in the transfected cells, but not in the wildtype cells. Both cell types also show bands at around 43 kDa. Exposure time: 40 s. Uncropped blot in Supplementary Material S5

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