Fig. 3 | Scientific Reports

Fig. 3

From: Fluorescence Guided Raman Spectroscopy enables the training of robust support vector machines for the detection of tumour marker proteins

Fig. 3

Comparison of Raman spectra of Cx43-mTagBFP2 and mTagBFP2-ER5. (a) The average spectra of Cx43-mTagBFP2 and the control mTagBFP2-ER5 show distinct spectral peaks at the significance level of p ≥ 0.01. (b) Overview of the average Raman intensity (solid line) and its first standard deviation (shade) at known Raman shifts for Cx43-mTagBFP2, mTagBFP2-ER5, and their absolute difference. Raman shifts where differences exceeded the double standard deviation (dashed red line) are shaded in light grey. (c) Correlation of the brightfield and fluorescent images allows colocalization of the regions of interest for measurement (red line). autofl. indicates imaging based on green autofluorescence. White bar: \(\:\approx\:\)5 μm (d) Comparison of the peak intensity measured at known Raman shifts identifies significant changes for proteins. (n = 95, Bars extend between 25th and 75th percentile. White line indicates the median. Black dots are individual data points. Wilcoxon rank sum test * p < 0.05, **p\(\:{\le\:10}^{2}\), p\(\:{\le\:10}^{3})\).

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