Table 2 Assignment of Raman Peaks.

From: Fluorescence Guided Raman Spectroscopy enables the training of robust support vector machines for the detection of tumour marker proteins

Shift [cm–1]

Assignment

References

Protein

Nucleic Acids

Lipids

Others

480

   

Glycogen

64,71

720

 

C-N stretching Adenosine

C-N stretching

 

72

746/750

   

Cytochrome C

55,64,73

750/752–760

Ring breathing in Tryptophan

   

72,74,75

811

 

RNA: C5 -O-P-O-C3 phosphodiester bonds stretching

  

72

877

  

C-C-N+ symmetric stretching

Carbohydrate: C-O-H ring

72

1003

Phenylalanine ring-breathing

   

64,71

1081

 

PO2 − symmetric stretching

  

76

1090/1095

  

Cytosine, Guanine, Adenosine

 

17,72

1180

 

Cytosine, Guanine, Adenosine

  

72

1235–1240

Peak: β-pleated sheet; absence: α-helix

   

54

1295/1297/1300

  

CH2 vibrations

 

17,64,71

1337

In the Amide III region

   

71

1449/1452

CH2 / CH3 vibrations

 

CH2 / CH3 vibrations

 

71,76

1580

High wavenumber end of Amide II (1550-1580)

   

77

1582

   

Cytochrome C

55

1605

   

Retinoic Acid

78

1654–1655

Amide I: C=O stretching mode, peptide linkage

   

71,72,76

1660

  

C-C stretching

 

64,72

  1. Raman shifts and their respective chemical groups for proteins, nucleic acids, lipids, and other biological substances.
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