Fig. 4

Micro dilution assay for minimal inhibitory concentration (MIC) determination. Methicillin Resistant Staphylococcus aureus (MRSA), Methicillin Sensitive Staphylococcus aureus (MSSA) was with MAgNPs (A and B), and MLE (C and D), before incubation (A and C), after 20 h of incubation (B and D). In each 96-well plate, column 11 contained bacteria only (positive control) and column 12 contained media only (negative control). (A) MRSA and MSSA treated with MAgNPs using serial dilution at concentrations of 0.441 mg/mL to 0.00043 mg/mL at the start of the experiment (0 h). (B) MRSA and MSSA treated with MAgNPs after 20 h of incubation at 37 °C. (C) MRSA and MSSA treated with MLE using serial dilution at concentrations of 32 mg/mL to 0.06 mg/mL at the start of the experiment (0 h). (D) MRSA and MSSA treated with MLE after 20 h of incubation at 37 °C.