Fig. 4 | Scientific Reports

Fig. 4

From: Ciprofol suppresses glycolysis and EMT in colorectal cancer cells by activating APC to modulate the Wnt/β-catenin signaling pathway

Fig. 4

Relief of Ciprofol’s Inhibitory Effects on HCT116 Cell Glycolysis and Invasion upon APC Protein Downregulation. (A) The relative expression level change of APC mRNA in HCT116 cells was detected by qPCR assay 48 h after transfection with siAPC—infected RNA sequence. (B) The relative expression level of APC protein in HCT116 cells was detected by Western blot assay. (C) Transwell experiment assessing changes in cell invasion ability. (D) QPCR analysis of HK2 and LDHA mRNA expression levels. (E) Measurement of ATP levels, lactate production, and glucose metabolism in HT29 cells treated with ciprofol. (F)Measurement of HT29 cell ECAR (extracellular acidification rate) and OCR (oxygen consumption rate) at different time intervals. (G) Western blot analysis of E-cadherin, N-cadherin, MMP2, and MMP9 protein expression levels. (H) Western blot analysis of HK2, LDHA and PKM2 protein expression levels. (*indicates statistical significance compared between the two groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

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