Fig. 5

Derivative83 Counteracts Ciprofol’s Inhibition of Wnt/β-Catenin Signaling in HT29 Cells. Transwell experiment assessing changes in cell invasion ability. (B) Western blot analysis of p-GSK3β, GSK3β, and β-catenin protein expression levels. (C) QPCR analysis of HK2 and LDHA mRNA expression levels, and measurement of ATP levels, lactate production, and glucose metabolism in colorectal cancer cells. (* indicates statistical significance compared between the two groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).