Fig. 4

Effects of phase duration of the electrical stimulation on the activation ratio. (a) An illustration of the activation ratio in a LE rat under 1 Hz stimulation with different phase durations (500, 1000, and 1500 µs) at 200 µA. The activation ratio was defined as the ratio of the activated area (red part) to the total area (including the activated and the inactivated area). (b) and (c) depict the activation ratio elicited by varying stimulation parameters (1, 5, and 10 Hz electrical stimulation with different phase durations) when receiving threshold and suprathreshold current, respectively, in the LE group (16 trials, 8 rats). (d) and (e) present similar data as in (b) and (c), respectively, for the F1 groups (8 trials, 4 rats). Multi-factor mixed ANOVA with Tukey post-hoc comparison was used for statistical analysis (*p < 0.05, **p < 0.01). The error bars represent the standard error of the mean.