Table 4 Oligonucleotide sequence of primers used in the RT-PCR analysis.

From: Modulation of microbiota, inflammation, iron status and gene expression of affected receptors in Parkinson’s disease rat model by synbiotic and dark chocolate

Primer name

Primer sequence 5′–3′

Annealing temperature (°C)

Amplicon size (bp)

References

Bacteroidetes F

GTTTAATTCGATGATACGCGAG

58

122

51

Bacteroidetes R

TTAASCCGACACCTCACGG

Firmicutes F

GGAGYATGTGGTTTAATTCGAAGCA

57

126

52

Firmicutes R

AGCTGACGACAACCATGCAC

Lactobacillus F

GGAATCTTCCACAATGGACG

60

217

53

Lactobacillus R

CGCTTTACGCCCAATAAATCCGG

  1. F: Forward primer, R: Reverse primer, bp: base pair.
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