Fig. 2 | Scientific Reports

Fig. 2

From: Exposure of cells to near-infrared irradiation relaxes chromatin compaction and facilitates recognition of cyclo-butane pyrimidine dimers

Fig. 2

CPD removal is dependent on the nIR dose and on incubation temperature. (A) Immuno-slot plot quantification of the removal of CPDs after combined UVB and nIR exposure. Cells were exposed to a constant dose of 360 J/m2 UVB combined with varying doses of nIR. The resulting band intensities were normalized to the band intensity directly after the exposure and fitted with a single exponential function. Plotted are the mean values of at least three independent experiments, the error bars represent the standard error of the mean. (B) Removal of CPDs when irradiation and subsequent incubation is carried out at 4 °C. The solid lines represent the measured band intensities again normalized to the values obtained directly after irradiation. No decline in band intensity and no effect of co-exposure with nIR can be observed. The data points show the mean values of at least three replicates and the error bars represent the standard error of the mean. Curves are fitted single exponential decay curves.

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