Fig. 5 | Scientific Reports

Fig. 5

From: Exposure of cells to near-infrared irradiation relaxes chromatin compaction and facilitates recognition of cyclo-butane pyrimidine dimers

Fig. 5

Colony formation and HPRT mutations after UVB, nIR and the combination of UVB and nIR in HaCaT cells. (A) Mutation frequency assessed by the colony formation after exposure of the cells to HAT medium (hypoxanthine-aminopterin-thymidine). Ten plates per irradiation condition were scored (grey dots) and are represented as box plots. Stars indicate significant differences using t-tests statistics: ** p < 0.01; *** p < 0.0001. (B) Colony formation assay for UVB (purple), nIR (green) and the combination (orange) for a dose range of up to 1.44 kJ/m2 UVB and 600 kJ/m2 nIR. All data points are normalized to the time matching controls. Points represent the mean of at least 5 plates from three biological replicates. Error bars represent the standard error of the mean. The data points were fitted with a linear regression.

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