Fig. 1

Effect of cell wall stress on total mRNA concentration [mRNA], global synthesis rate (SR), and degradation constant (kD). Cells growing exponentially in YEPD were collected at time 0, then exposed to Congo red, and aliquots were collected at 30, 60, 120, 180, and 240 min to measure [mRNA], SR and kD. See “Methods” for details. The parameters are expressed relative to their respective time 0 values (defined as the unit). kD values above 1 indicate mRNA destabilization, while values below 1 indicate stabilization. The mean ± SD from three independent experiments are shown.