Fig. 3
mRNA half-lives of the KDX1, APE1, CRH1, and SRL3 genes align with GRO experiment predictions. (a) Representation of kD kinetics for each gene from GRO experiment. (b) mRNA half-lives (t1/2) of selected transcripts were determined using the thiolutin (3 µg/mL) transcriptional shut-off method (see “Methods” for details) in the wild-type strain after 2 h of growth in the presence or absence of Congo Red (CR, 30 µg/mL). The mean ± SD from three independent experiments is shown. The arrow in left panels marks 120 min time when the thiolutin experiments on right panels were done.
