Fig. 6

Identification and validation of miRNAs in LELNs by small RNA high-throughput sequencing. (A) Length distribution of miRNAs in LELNs, ranging from 18 to 30 nucleotides. (B) Top eight miRNAs with the highest read counts, ranked in descending order. (C) Top four predicted novel miRNAs with the highest read counts. (D) Sequences of the 12 miRNAs identified through sequencing. (E) Validation of the read count results for 12 selected miRNAs using RT-qPCR (n = 3).