Fig. 2 | Scientific Reports

Fig. 2

From: Dermal papilla cells-conditioned medium attenuates oxidative stress-induced senescence via ferroptosis inhibition

Fig. 2

UVA promoted cell senescence by regulating ferroptosis. (A) CCK-8 assay results showed the viability of mouse skin fibroblasts 48 h after treatment. Groups: NC (normal fibroblasts), UVA (6 J/m2 UVA irradiation), and UVA + Fer-1 (2 μM ferrostatin-1 with 6 J/m2 UVA). (B) SA-β-Gal staining of mouse skin fibroblasts senescence, scale bar = 100 μm. (C, D, and E) Biochemical detection of Iron, GSH, and ROS levels, scale bar = 200 μm. F. Western blot analysis of ferroptosis-related pathway proteins NRF2, GPX4, and SLC7A11, and relative expression levels were quantified based on band intensity normalized to β-actin. *P < 0.05, **P < 0.01, ***P < 0.001.

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