Fig. 4 | Scientific Reports

Fig. 4

From: Gata3 drives miR-21-5p transcription to mitigate hyperoxia-induced lung injury, independent of CpG Island methylation

Fig. 4

Gata3 promotes miR-21-5p expression to reduce AECs apoptosis. (A) Bar graph of Gata3 RNA overexpression efficiency. (B) WB analysis of Gata3 protein bands. (C) Statistical analysis bar chart of protein band in (B). (D) Bar graph of miR-21-5p expression after Gata3 overexpression. (E) ChIP analysis bar graph. (F) Bar graph of miR-21-5p knockdown efficiency using inhibitor. (G) Statistical bar chart of miR-21-5p expression in Gata3 and inhibitor overexpression experiments. (H) WB bands of apoptosis-related proteins in Gata3 and inhibitor overexpression experiments. (I) Statistical analysis bar chart of protein band in (H). (J) Flow cytometry analysis of apoptosis rate in TC-1 cells. (K) Statistical bar chart of apoptosis rate from flow cytometry analysis. *P < 0.05, **P < 0.01, ***P < 0.001.

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