Fig. 5 | Scientific Reports

Fig. 5

From: Gata3 drives miR-21-5p transcription to mitigate hyperoxia-induced lung injury, independent of CpG Island methylation

Fig. 5Fig. 5

Gata3 alleviates AECs apoptosis and lung injury in WT but not in KO mice. (A) Fluorescence images showing SPC-GFP-Gata3 adenovirus infection efficiency via tracheal instillation. (B) Bar graph of Gata3 RNA overexpression. (C) WB showing Gata3 protein overexpression. (D) Statistical bar graph of protein band intensity in (C). (E) Bar graph of miR-21-5p expression after Gata3 overexpression. (F) miR-21-5p expression in WT and KO mice after Gata3 overexpression and HALI modeling. (G) WB showing apoptosis-related protein expression in WT and KO mice following Gata3 overexpression and HALI modeling. (H) Statistical analysis of apoptosis-related protein expression in (G). (I) Fluorescence images of Cleaved-caspase3 expression and HE staining in lung tissue from WT and KO mice after Gata3 overexpression and HALI modeling. (J) Statistical bar graph of Cleaved-caspase3 fluorescence intensity in (I). (K) Statistical bar graph of lung tissue injury in (I). (A) scale bar = 1000 μm; (I) scale bar = 100 μm; *P < 0.05, **P < 0.01, ***P < 0.001.

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