Fig. 3

Preincubation with Plant Extract Reduced Inflammation Induced by S. aureus. NHEKs were preincubated with vehicle for 24 h and then treated with the bacteria for 3 h in absence (S. aureus) or in presence of the extract (Simult. Inc. extract + S. aureus). NHEKs were also preincubated with the plant extract for 24 h and then treated with the bacteria in absence of the plant extract for 3 h (Preinc. Extract + S. aureus). Then, gentamycin was added and incubation was continued for 24 h. Gene expression levels of (a) IL-8, (b) IL-6 (c) CCL5 and (d) filaggrin were determined by real-time PCR. Statistical significance was tested by One-way ANOVA test with subsequent Bonferroni’s multiple comparison test. Bars are means  ± SEM of seven experiments performed in triplicates (n = 7; *p < 0.05; **p < 0.01; ***p < 0.001; ns = not significant).