Fig. 5
From: Atopic dermatitis-related anti-inflammatory in vitro effects of a plant extract mixture
Impact of AhR siRNA-Mediated Knockdown on the Effects of the Plant Extract in the 2D AD Model. NHEKs were transfected with AhR siRNA or control siRNA. 48 h later, NHEKs were stimulated with the AD-associated cytokine mixture (IL-13, IL-4, IL-22 and TNF-alpha, each 10 ng/mL) either in the absence (AD) or in the presence of the plant extract (AD + Extract). Gene expression levels of (a) CYP1A1 (b) filaggrin (c) TNFα (d) CA2 and (e) CCL26 were determined by real-time PCR. Statistical significance was tested by unpaired t-test (n = 3; *p < 0.05; **p < 0.01; ns = not significant). Bars indicate means ± SEM.
