Fig. 2 | Scientific Reports

Fig. 2

From: Fiber-specific differences in protein content of pathways related to mTORC1 signaling and oxidative metabolism in individuals with obesity

Fig. 2

Total protein content of akt-1, mTOR, p70S6K, and S6RP of whole muscle homogenate (A-D) and in isolated type I and IIa muscle fibers (E-H) from obese (green) and healthy (orange) individuals. The membranes with whole muscle homogenate samples were cut into three sections at 100 kDa for mTOR; at 50–100 kDa range for p70S6K and β-tubulin; and under 50 kDa, for S6RP proteins. Because Akt-1 and β-tubulin have similar molecular weights, two gels were prepared in duplicate and run simultaneously to use β-tubulin as a loading control. For the membranes loaded with specific fiber samples, they were also cut into three sections at 100 kDa, for mTOR and MHC; at 50–100 kDa range, for Akt-1 and p70S6K; and under 50 kDa, for S6RP proteins. * : P < 0.05.

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