Fig. 1 | Scientific Reports

Fig. 1

From: Transcriptomic and electrophysiological alterations underlying phenotypic variability in SCN1A-associated febrile seizures

Fig. 1

Generation and characterization of forebrain neurons from iPSC lines. (A–B) Bright-field images of forebrain neurons differentiated from iPSCs at the early (A) and the late (B) stages of differentiation. (C–E) Immunofluorescence staining confirms successful neuronal differentiation, as indicated by the expression of specific neuronal markers: MAP2 and NEFH (C), NEFL (D), and TUBB3 (E). (F) Quantification of TUBB3-positive cells demonstrates a high differentiation efficiency (~ 85–90% at the early stage and ~ 100% at the late stage) across all iPSC lines. Data are presented as mean ± S.E.M., with each data point representing an individual analyzed image.

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