Fig. 6
Analysis of AMPA/GABA current by voltage clamp. (A) AMPA/GABA current ratio (expressed as percentage ± S.E.M.) of 20 μM AMPA- and 500 μM GABA-evoked currents at the early stages of differentiation. The AMPA/GABA current ratio was significantly different between the two phenotypes (blue filled bar: 291.4 ± 29.5%, n = 11; versus green filled bar: 567.6 ± 73. 6%, n = 10; p = 0.002, t-test). The inset on the right shows the GABA- and AMPA-evoked current traces. (B) AMPA-evoked current (percentage ± S.E.M.) blocked by 50 μM IEM 1460 (a selective blocker for GluA2-lacking receptors) in HC and SEV phenotypes (blue filled bar: 63.9 ± 7.2%; n = 10; vs green filled bar: 75.1 ± 2.6%; n = 16; p = 0.128, t-test). The inset on the right shows AMPA-evoked currents recorded from samples with IEM 1460 application (50 μM, black bar) to block GluA2-lacking AMPAR currents. IEM 1460 inhibition was tested using a holding potential of − 80 mV.
