Fig. 7
Zn2+ selectively inhibits γ-lacking extrasynaptic GABAARs. (A) Ratios of GABRA3 to GABRG2 (α3/γ2), showing a lower ratio in SEV neurons compared to HC. Conversely, (B) the ratios of GABRA3 to GABRB3 (α3/β3) and (C) GABRA3 to GABRD (α3/δ) are higher in SEV neurons, indicating a more immature state relative to HC lines. (D) Percentage decrease in GABA current ± S.E.M. following treatment with 40 μM Zn2+ (blue filled bar: − 32.2 ± 4.9%; n = 14; vs green filled bar: − 53.1 ± 3.6%; n = 17; p = 0.004, t-test). The inset on the right shows the 200 μM GABA-evoked current before and after treatment with 40 μM Zn2 + (black traces) for both HC and SEV neurons.
