Fig. 2 | Scientific Reports

Fig. 2

From: Enhanced activity localization and microscale dosimetry in alpha-emitter radiopharmaceutical therapy using integrated autoradiography and histological imaging

Fig. 2

The extent of freezing damage on kidney tissue stained with phalloidin when cryopreserving by: (A) placing embedded tissue on dry ice, resulting in extensive damage causing ripped tissue, with no discernable tubular structure left (detail in D); (B) using − 20 freezer or Peltier element in cryostat, further exacerbating the observed freezing damage (detail in E); (C) Snap-freezing in isopentane chilled in liquid nitrogen, preserving the morphology of glomeruli and tubules (detail in F). (G) Major vasculature is readily discernable with phalloidin stain of snap-frozen kidney tissue. (H) P&H stain false-colored to look like a normal histological H&E stain.

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