Fig. 3
From: Thiostrepton induces apoptotic cell death at the level of BCL-2/CED-9 in C. elegans
Influence of p53 Activity on Thio-Induced Apoptosis in C. elegans Mutants a Representative DIC, GFP, and merged images of adult germlines from ced-1::gfp; cep-1(lg12501) double mutant worms treated with DMSO or 180 µM Thio for 24 h. Thio induces apoptosis in the cep-1 loss-of-function background, indicating that Thio-induced apoptosis is independent of CEP-1/p53 protein. Scale bar = 20 μm. b Quantification of apoptotic germ cell corpses per gonad in ced-1::gfp, and ced-1::gfp; cep-1(lg12501) double mutants following DMSO or 180 µM Thio treatment. Bars represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test; ns = not significant. n > 50 germlines per condition. c Representative DIC images of adult germlines from wild-type and egl-1 (LoF) mutants treated with DMSO or 180 µM Thio. Apoptotic corpses (yellow arrowheads) are visible following Thio treatment in both genotypes. d Quantification of germ cell corpses in wild-type and egl-1(LoF) strains after DMSO or 180 µM Thio treatment. Bars represent mean ± SEM. No significant difference was observed in the absence of EGL-1, indicating that Thio-induced apoptosis occurs independently of EGL-1. Statistical analysis was performed using one-way ANOVA (ns = not significant). n > 50 germlines per condition.
