Fig. 4
From: Thiostrepton induces apoptotic cell death at the level of BCL-2/CED-9 in C. elegans
Thio Does Not Induce Activation of the ATM/ATR Pathway in C. elegans Germline. a Immunostaining of dissected germlines from worms treated under the following conditions: Blank (untreated), DMSO, 180 µM Thio, and 25 mM hydroxyurea (HU; positive control). Germlines were stained with a phospho-ATM/ATR substrate motif antibody (red), which specifically detects proteins phosphorylated on (S/T) QG motifs, substrates of ATM/ATR kinases. DAPI (blue) marks nuclei. As expected, HU-treated worms displayed robust focal activation of ATM/ATR signaling, while no such activation was observed under Thio or DMSO conditions. b Zoomed-in of the mitotic region from panel a (dashed box). White arrowheads indicate distinct nuclear phospho-ATM/ATR-positive staining observed only in 25 mM HU-treated samples; however, no such phospho-ATM/ATR substrate staining was detected in Blank, DMSO, or Thio-treated germlines. Scale bars = 20 μm.
