Fig. 1
From: Method development for correlating lipid molecular information with anatomy in C. elegans
Embedding and sectioning optimization. (a) Schematic of the sample preparation workflow. (b) Photographs of nematodes trapped in the PDMS chip and (c) embedded in gelatin after removal of the PDMS chip. Red arrows indicate nematodes. (d) 3D printed mould and lid. (e) Frozen sample block secured onto a cryotome specimen disc. (f) Glass slide with gelatin sections. A nematode (white line in the air pocket) is indicated by a yellow arrow. Inset: close-up view of the nematode. (g) Confirmation of the location and preservation of the sections using hematoxylin staining. (h) Representative microscopy images defining the fragmentation rankings. The rankings are based on the number of fragments produced: low = 1–2 fragments, low/moderate = 3–4 fragments, moderate = 5 fragments, moderate/severe = 6–7 fragments, severe = 7+ fragments. (i) Comparison of the fragmentation of nematode sections and j) ratio of nematode to air pocket for the two embedding media. Bars in (j) show the standard error (SE) and correspond to \(\pm 1 SE\).
