Fig. 3
From: Method development for correlating lipid molecular information with anatomy in C. elegans
Average MS intensity variations in nematodes and consecutive sections. Schematics showing the differences between the data collection methods used to obtain the datasets a) “N2 all” and b) “Nematode 1–4”. (a) Twelve consecutive gelatin block sections containing 5 different nematodes. (b) Consecutive sections obtained from a single nematode. “Nematode 1-3” in (a) represent data points in “N2 all”, while “Consecutive section 1–3” in (b) correspond to separate data points in “Nematode 1-4”. (c) Absolute intensity of the MS peak at m/z 423.31 for the datasets “N2 all” and “Nematode 1–4”. Intensities were obtained from average nematode ROI spectra in arbitrary units. The bars correspond to \(\pm 1 SE\). (d) MS spectra of a representative nematode from the “N2 all” dataset and its gelatin background, with matrix peaks m/z 167.08 and m/z 333.13, and a lipid signal at m/z 423.31.
