Fig. 6
From: Method development for correlating lipid molecular information with anatomy in C. elegans
Image reconstruction and 3D visualisation. (a) Schematic overview of feature-based reconstruction. Yellow circles represent the selected feature and location used for reconstruction, while the numbers in the left and right image represent the matching features in the nematode graphics. (b) Optical image used as a template for feature-based reconstruction. Structures used as markers are labelled with yellow numbers (an example is circled in red). (c) Target image for reconstruction based on the template (b). Matching structures are labelled with yellow numbers with an example circled in red. Inset: corresponding MSI image. (d) Resulting reconstructed image. Inset: corresponding MSI image. (e) Misaligned stacked 3D images of consecutive sections. (f) Stacked 3D reconstructed images. (g) Stacked 3D MSI images after feature-based reconstruction.
