Fig. 2

Activation of CA3 and S1 astrocytes by WES of the MS/vDB in basal state. (a) Timeline of calcium signals collection in the WES-base group. (b) Calcium signals from astrocytes of CA3 and S1 were recorded 1/6 h before WES-base, during WES-base, 1/6 h, 1 h, 12 h, 1, 3, 5, 7 and 10 days after WES-base. Synchronized calcium signals of CA3 and S1 astrocytes indicated by gray rectangles. (c) Probability of synchronized calcium signals of CA3 and S1 astrocytes 1/6 h before WES-base, during WES-base, 1/6 h, 1 h, 12 h, 1, 3, 5, 7 and 10 days after WES-base. (d) Amplitude and frequency of calcium signals of CA3 astrocytes 1/6 h before WES-base, during WES-base, 1/6 h, 1 h, 12 h, 1, 3, 5, 7 and 10 days after WES-base. (e) Same as in d for S1. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. One-way ANOVA followed by Dunnett’s multiple comparisons test for (c–e).