Fig. 4

Statistical outcomes of WES of the MS/vDB on SE at different phases of astrocytic Ca²⁺ and accompanied by EEG and chronic epileptic model validation. a-h, Effects of WES of the MS/vDB at different phases on number of GSs (a), The latency to 1st-GS (b), Incidence of calcium wave events occurring simultaneously in two brain regions or in a single brain region during GS (c), Change in the latency of the CA3 wave relative to the S1 wave during GS (left) and change in the latency of the GS relative to the S1 wave during GS (right) (d), Incidence of combinations of waves occurring simultaneously in two brain regions or a single brain region during GS (e), Number of wave4 in CA3 (f), Duration of GS (g), Total duration of GS (h). (i–k) Effects of WES of the MS/vDB at different phases on number of FSs (i), Incidence of calcium wave events occurring simultaneously in two brain regions or in a single brain region during FS (j), Incidence of combinations of waves occurring simultaneously in two brain regions or a single brain region during FS (k). (l) Representative EEG traces recorded after KA i.p.. (m–o) Effects of WES of MS/vDB on number of GSs (sham + PILO, n = 5; PILO-WES, n = 6) (m), Number of FSs (n), Maximum seizure stage (o). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Chi-square test for (c,e,j,k); Unpaired t-test for (a,b,d,f–i,m–o).