Fig. 6
From: Inhibiting astrocyte calcium dynamics terminates status epilepticus via wireless septal stimulation
The role of the astrocyte-pyramidal neuron interaction in the CA3 microcircuit. (a) Scheme of experiments of hM3Dq expression in CA3 astrocyte for chemogenetic activation. (b) Representative sagittal image of hM3Dq expression in CA3 astrocyte and GCaMP6(f) expression in CA3 and S1 PN. Left, Scale bar, 100 μm. Right, Scale bar, CA3, 50 μm. S1, 100 μm. (c) Timeline of calcium signals collection in GfaABC1D-hM3Dq and GfaABC1D-mCherry groups. (d) Representative calcium signals (ΔF/F) of CA3 and S1 PNs 90 min after KA i.p. in the GfaABC1D-mCherry group (n = 5). Black rectangular box indicates CSD occurrence in S1 and and corresponding CA3 PNs calcium signals (ΔF/F). The horizontal dashed line represents the baseline level of CA3 and S1 calcium signals. Unidirectional solid arrow indicates the onset of flashing in CA3 and S1. (e) Enlarged views of CA3 and S1 PN calcium signals (ΔF/F) inside the black rectangular box of d. (f) Enlarged views of calcium signals flashing (ΔF/F) in CA3 and S1 PNs. (g) Representative calcium signals (ΔF/F) of CA3 and S1 PNs 90 min after KA i.p. in the GfaABC1D-hM3Dq group (n = 5). Gray shading indicates GS occurrence. The horizontal dashed line represents the baseline level of CA3 and S1 calcium signals. Unidirectional solid arrow indicates the onset of flashing in CA3 and S1. (h) Enlarged views of CA3 and S1 PN calcium signals (ΔF/F) inside the Gray shading of g and corresponding heatmap. The horizontal dashed line represents the baseline level of CA3 and S1 calcium signals. The vertical dashed line represents the beginning and end of GS. Bidirectional solid arrows represent the amplitude of AHP-like event in CA3. (i–o) Effects of chemogenetic activation of CA3 astrocyte on survival and death in mice (i), Maximum seizure stage (j), Number of GSs (k), The latency to 1st-GS (l), Total duration of GS (m), The latency to calcium signals flashing in CA3 and S1 (n), The relationship between the latency to calcium signals flashing and latency to CSD (o). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Chi-square test for (i); Unpaired t-test for (j–n), Paired t-test for (o).
