Fig. 7
The microprocessor and RISC components exhibit altered subcellular localization in different cell densities. Immunofluorescence and confocal microscopy of ARPE-19 cells cultured in fully confluent (100% plate coverage) or sub-confluent (< 90% plate coverage) conditions, stained for PLEKHA7, DROSHA, DGCR8, AGO2, GW182, and co-stained for p120. DAPI is the nuclear stain. Merged images are shown on the right. Enlarged image insets are denoted with white boxes and shown to the right of their respected images. Magenta arrowheads indicate junctional localization. Scale bars are shown at the bottom right image and inset and are the same for all images and insets. Line scan quantifications spanning the nuclear, cytoplasmic, and junctional compartments per cell line are shown on the right column; the dark - colored lines represent the average fluorescence intensity of 15 cells from three fields for each cell line, whereas the light - colored areas represent standard deviations.
