Fig. 3

Reconstruction method related blocks. The CTF can for both, SSB (a–c) and ITR methods (h–j) be switched from multiplications of probe semi-angle to scattering angles or real space sampling. For ITR the sample plane charts dealing with real space probe density, corresponding beam overlap and related probe window can be found in geometrical (l) and simulated probe representation (k) in form of amplitude or intensity. Note: the geometrical beam diameter is computed from probe semi-angle, defocus and beam diameter in focus. The camera length guide (m) shows the important characteristics (reconstruction pixel size, minimal thickness of a slice for multislice reconstruction, detector coverage, probe window and overall sampling) with respect to nominal camera length and set checks which results can be found in (n). There is also a simplified version for SSB consisting of a sample plane chart (d, e) with recommended scanning step size (based on probe semi-angle related CTF) and maximum camera length enabling at least \(1\alpha\) on detector (f). Results of checks can be found in (g).