Fig. 3

Intracellular cytokine measurement by flow cytometry. (A) Percentage of CD3⁺CD4⁺ cells expressing interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α) and both cytokines; (B) Percentage of CD3⁺CD8⁺ cells expressing IFN-γ, TNF-α and both cytokines; (C) Ratio of CD3⁺CD4⁺ and CD3⁺CD8⁺ cells calculated from the percentage of CD4⁺ and CD8⁺ T cells; (D) Percentage of CD3⁺CD4⁺CD25⁺ cells expressing Foxp3 from dogs fed control diet and experimental diet. Bars correspond to mean plus standard error of the mean. ** P < 0.01.