Fig. 1
From: Cytomegalovirus-induced oncomodulation drives immune escape in glioblastoma
Characterization of CMV induced changes to murine glioblastoma cells in vitro. (A + E) GL261Luc2 mouse glioblastoma cells were infected using mCMV-Δm157mCherry. Red florescence indicated viral infection. No plaque formation was detected in GL261Luc2. (middle) NIH3T3 are shown as positive control. (right). No fluorescence was detected after previous irradiation of virus stocks using UV light. (p = 0.0024) (B) Viral copy number increase intracellular and (C) in supernatant (p = 0.0001). (D) mCMV-associated pp65 homolog antigen was detected using immune staining (E) Proliferation measurement of 3D cell cultures in ultra-low attachment plates. (F) The distribution of cells among the cycle phases 72 h after infection with mCMV at MOI 1 (S phase: p = 0.9973; G0/G1, G2: p = 0.0009 and p = 0.0013).
