Fig. 5

Effect of chemogenetic regulation of PV-INs on mouse behaviour. (A) Time in center decreased after the inhibition of PV-INs under physiological conditions (n = 10 from 10 mice per group, **P < 0.01 vs. CON and CON + hM4D groups), with no difference in total distance between the two groups (P > 0.05 vs. CON and CON + hM4D groups). (B) Time in light zone decreased after the inhibition of PV-INs in the physiological state (n = 10 from 10 mice per group, ***P < 0.001 vs. CON and CON + hM4D groups). (C) Acute stress model was established 14 days after virus injection, and behavioural monitoring of CNO activation was performed 15 min before intraperitoneal injection. (D) Time in center increased after the activation of PV-INs under acute stress conditions (n = 10 from 10 mice per group, **P < 0.001 vs. AS3d and AS3d + hM3D groups), and the total activity distance of the acute stress group did not differ from that of the control group (P > 0.05 vs. AS3d and AS3d + hM3D groups). (E) Time in light zone increased after the activation of PV-INs under acute stress conditions (n = 10 from 10 mice per group, ***P < 0.001 vs. AS3d and AS3d + hM3D groups). (F) Chronic stress model was established 14 days after virus injection, and behavioural monitoring of CNO activation was performed 15 min before intraperitoneal injection. (G) Time in center increased after the activation of PV-INs under chronic stress conditions (n = 10 from 10 mice per group, ***P < 0.001 vs. CS14d and CS14d + hM3D groups), and the total activity distance of the chronic stress group did not differ from that of the control group (P > 0.05 vs. CS14d and CS14d + hM3D groups). (H) Time in light zone increased after the activation of PV-INs under chronic stress conditions (n = 10 from 10 mice per group, ***P < 0.001 vs. CS14d and CS14d + hM3D groups). Data are presented as mean ± SEM.