Fig. 8

Representative histology and Immunofluorescence vocal fold images for E cadherin and Zo-1-staining. Hematoxylin and Eosin-stained coronal sections of murine vocal fold region (10X [Figures A–C, black scale bar represents 100 um] and 60X [middle panel, Figures D–F, black scale bar represents 100 um]). Black box labelled within images in the top panel (Figures A–C) represent the portion (vocal fold region) that is magnified at 60 X (middle panel, Figures D–F). Mid-membranous vocal fold region stained with E-cadherin and DAPI (60X-Figures G–I, white scale bar represents 100 um) reveal no protein expression of E-cadherin across the length of the mid-membranous vocal fold epithelium of animals in short and prolonged stress groups, compared to control group (60X, panels G–I, white scale bar represents 100 um). Similarly, mid-membranous vocal fold region stained with Zo-1 and DAPI (60X, panels J–L, white scale bar represents 100 um) reveal decreased expression of Zo-1 in short and prolonged stress groups, compared to control group.