Table 1 Quantitative PCR Primer Sequences.
From: Effects of psychosocial stress on laryngeal microbiology and epithelial barrier integrity
Primer | Accession # | FWseq | RVseq | Size | Animal |
|---|---|---|---|---|---|
IL1β | NM_008361.4 | 5′-GAAATGCCACCTTTTGACAGTG 3′ | 5′-TGGATGCTCTCATCAGGACAG 3′ | 116 | Mouse |
TNF alpha | Y00467.1 | 5′-CTGTAGCCCACGTCGTAGCA 3′ | 5′-TGTGGGTGAGGAGCACGTA 3′ | 198 | Mouse |
IL6 | BC138766.1 | 5′-GTCCTTCCTACCCCAATTTCC 3′ | 5′-GGTCCTTAGCCACTCCTTCTG 3′ | 72 | Mouse |
MUC2 | NM_023566.4 | 5′-GTCCTGACCAAGAGCGAACA 3′ | 5′-ACAGCACGACAGTCTTCAGG 3′ | 103 | Mouse |
TLR2 | NM_011905.3 | 5′-AAACCTCAGACAAAGCGTCA 3′ | 5′-CACACCCCAGAAGCATCACA 3′ | 138 | Mouse |
TLR4 | NM_021297.3 | 5′-AGATCTGAGCTTCAACCCCTTG 3′ | 5′-ATTGTTTCAATTTCACACCTGGA 3′ | 102 | Mouse |
β -actin | NM_007393.5 | 5′-AGAGGGAAATCGTGCGTGAC 3′ | 5′-CAATAGTGATGACCTGGCCGT 3′ | 138 | Mouse |
