Table 8 Analytical methods employed and their lower limits of detection (LLOD).

Method (Units)	Reagent provider	Sample	LLOD	Reference
Stress biomarkers
AA (IU/mL)	a-Amylase, OSR6182, Beckman^a	Saliva Serum	2.0 × 10⁻³	²²
BChE (IU/L)	Colorimetric in-house method	Saliva Serum	2.5	⁹
Immune system
ADA (IU/mL)	Dyazime^b	Saliva Serum	0.07 × 10⁻³	¹³
IgG (mg/dL)	OSR6145, Beckman	Saliva Serum	0.0	²³
Inflammation
CRP (µg/L)	AlphaLISA based in-house method	Saliva	0.86	²⁴
CRP (mg/L)	Beckman	Serum	0.97	²⁵
Hp (mg/L)	AlphaLISA based in-house method	Saliva Serum	22.5 × 10⁻³	²⁶
Proteins (mg/dL)	protein in urine and CSF, Spinreact^c	Saliva Serum	0.1	⁵
MPO (IU/L)	Colorimetric in-house method	Saliva Serum	0.0	¹⁴
Ferritin (µg/L)	Tina-quant ferritin, Roche Diagnostics^d	Saliva Serum	1.49	²⁷
Redox status
AOPP (µmol/L)	Colorimetric in-house method	Saliva Serum	3.83	²⁸
UA (mg/dL)	Beckman	Saliva Serum	0.08	²⁸
FRA (µmol/L)	Colorimetric in-house method	Saliva Serum	1.46 × 10⁻⁵	²⁸
Tissue damage
AST (IU/L)	Beckman	Saliva Serum	1.0	²⁹
CK (IU/L)	Beckman	Saliva Serum	3.0	²⁹
LDH (IU/L)	Biosystem^e	Saliva Serum	3.0	²⁹
Routine
ALP (IU/L)	Beckman	Saliva Serum	1.0	⁵
GGT (IU/L)	Beckman	Saliva Serum	1.0	⁵
Urea (mg/dL)	Beckman	Saliva Serum	0.78	⁵
Triglycerides (mg/dL)	Beckman	Saliva Serum	1.0	³⁰
Calcium (mg/dL)	Beckman	Saliva Serum	0.12	⁵
Phosphorous (mg/dL)	Beckman	Saliva Serum	0.31	^[5

AA: α-amylase; BChE: butyrylcholinesterase; ADA: adenosine deaminase; IgG: immunoglobulin G; CRP: C-reactive protein; Hp: haptoglobin; MPO: myeloperoxidase; AOPP: advanced oxidation protein products; UA: uric acid; FRA: ferric reducing ability; AST: aspartate aminotransferase; CK: creatin kinase; LDH: lactate dehydrogenase; ALP: alkalin phosphatase; gGT: γ-glutamil transferase.
^aBeckman Coulter Inc., Fullerton, CA, USA. ^bDiazyme Laboratories, Poway, CA, USA. ^cSpinreact, Barcelona, Spain. ^dRoche Diagnostics, Indianapolis, IN, USA. ^eBiosystem S.A., Barcelona, Spain.

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