Fig. 1

A. Volcano Plot: Each point in the figure represents a gene, and the top 5 up-regulated genes and top 5 down-regulated genes are marked according to the log2FC order of fold change; B. Distribution as Heatmap: Each column represents a sample, and the top 10 up-regulated and top 10 down-regulated genes ranked by fold change log2FC are plotted in this figureC1. Venn Diagram: DEGs vs. MRGs: The numbers indicate the count of genes in each category and their intersections. C2. Venn Diagram: DEGs vs. NETs-RGs. D1. Kaplan-Meier survival analysis between high and low score groups of intersection genes in C1 figure. D2. Kaplan-Meier survival analysis between high and low score groups of intersection genes in C2 figure. E. Sample level clustering: Hierarchical clustering was performed using the Euclidean distance of the expression to check the absence of outliers in the samples. F. Soft threshold: From the left figure, when the scale-free fitting index is 0.85, the minimum soft threshold conforming to the construction of the scale-free network is 5, and the right figure shows the network connectivity under different soft thresholds. G. Cluster Dendrogram: Dendrogram resulting from hierarchical clustering of genes. The branches indicate groups of genes sharing comparable expression profiles, 22 modules were obtained. H. Correlation heat map: The color bar on the left indicates different gene modules, while the right color bar shows the correlation scale. In the central heat map, darker colors correspond to stronger correlations, with red denoting positive and blue indicating negative correlations. Numbers within each cell represent correlation coefficients and statistical significance.