Fig. 3 | Scientific Reports

Fig. 3

From: Establishment and rescue of fibroblast cell lines carrying a nonsense mutation of RB1 by CRISPR-based base editing

Fig. 3

RB1 gene disruption changes cell behavior and regulation. (A) Expression level of cell cycle regulation-engaged genes in WT and R552X cells measured by qPCR from nine independent experiments (n = 9; mean ± SEM). The average expression level of WT cells was normalized to 1, and the expression levels of He- and Ho-R552X were adjusted accordingly. (B) Relative cell proliferation rate. Cell expansion rate of each cell line was calculated using 15 independent measurements (n = 15; mean ± SEM) up to day 3. (C,D) Measurement of cell cycle progression changes in RB1-disrupted cell lines. Each cell line was stained with propidium iodide and each phase in the cell cycle were analyzed proportionally using flow cytometry across four independent experiments (n = 4; mean ± SEM). P values were obtained using one-way analysis of variance (ANOVA) and Tukey’s test for post hoc analysis: *P < 0.05, **P < 0.01, ***P < 0.001.

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