Fig. 2 | Scientific Reports

Fig. 2

From: M2-like macrophages derived from THP-1 cells promote myofibroblast differentiation of synovial fibroblasts in association with the TGF-β1/SMAD2/3 signaling pathway

Fig. 2

Characterization of THP-1-derived M1- and M2-like macrophages. (A) Representative images of different morphologic appearances of THP-1 cells and M0, M1-, and M2-like macrophages. Magnification, ×100. Scale bar = 100 μm. (B, C, and D) Flow cytometry was used to analyze the percentages of positive cells using specific markers of (B) macrophages (CD14) and (C) M1 (CD80) and M2 (CD206) macrophages. The left panels are representative flow cytometry histograms. The right panels present the percentages of positive cells. (D) Representative flow cytometry plots of M1-like (CD14+, CD80+) and M2-like (CD14+, CD206+) macrophages. (E) Gene expression levels of M1 and M2 macrophage markers measured by RT-qPCR. Data are presented as the mean ± SEM from three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001.

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