Fig. 3

M2-like macrophages induce myofibroblast differentiation of HSFs. (A) Gene expression analysis of α-SMA using RT-qPCR. Data are expressed as individual data points and mean values. (B) Percentages of α-SMA positive cells by flow cytometry after co-culture for 72 h. Data are averages of three independent experiments and presented as the mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001. (C) Representative flow cytometry histograms.