Table 1 Experiment overview. Rumen fluid batches were collected weekly, shipped overnight and stored on ice before being subdivided for the individual experiments. Two types of experiments were performed: compound tracing (T); and dose-response (D). Single inhibitors were added to each experiment: bromoform (BF), dibromomethane (DBM), bromomethane (BM) and Asparagopsis taxiformis (A. Tax).
From: The antimethanogenic efficacy and fate of bromoform and its transformation products in rumen fluid
Rumen fluid batch | Experiment name | Storage duration (h) | Experiment type | Incubation time (min) | Added inhibitor |
---|---|---|---|---|---|
1 | Exp1 | 48 | T | 260 | BF |
Exp2 | 72 | T | 260 | DBM | |
2 | Exp3 | 48 | D | 306 | A.Tax |
Exp4 | 72 | T | 260 | A.Tax | |
3 | Exp5 | 48 | D | 285 | BF |
Exp6 | 72 | D | 240 | DBM | |
Exp7 | 96 | D | 345 | BM |