Fig. 1
From: Detecting MUNC18-1 related presynaptic dysfunction and rescue in human iPSC-derived neurons
Properties of NGN2 neurons characterized with whole-cell patch clamp recordings. (A) Both 3-week (n = 21) and 6-week (n = 18) neurons displayed evoked action potential firing with increasing current injection. Left panel: percentage of neurons with evoked action potentials. Right panel: repetitive action potential firing in response to current steps from 0 to 100pA. (B) Example traces of evoked action potential firing. (C) An increase in the percentage of cells showing spontaneous firing out of all cells sampled from week 3 to week 6 demonstrating maturation. (D) Example traces of spontaneous firing in 3-week and 6-week neurons. (E) An increase in sEPSC frequency but not amplitude in 6-week neurons vs. 3-week neurons demonstrating presynaptic maturation. (F) Example traces of sEPSCs. *p < 0.1, Student’s t-test. Error bars represent mean ± SEM.
