Fig. 3
From: Detecting MUNC18-1 related presynaptic dysfunction and rescue in human iPSC-derived neurons
Patch clamp and MEA recording demonstrating synaptic phenotype of STXBP1 KO NGN2 neurons. (A) Illustration of editing on the STXBP1 gene to generate STXBP1 knockout hiPSC line. (B) Quantification of STXBP1 protein with automated western blot system. (C) Whole cell voltage clamp recording of miniature EPSC frequency in STXBP1 ISO control (n = 13) vs. KO NGN2 neurons (n = 5). (D) Example traces of mEPSCs in STXBP1 ISO control and KO NGN2 neurons. (E) MEA recording of STXBP1 ISO control and KO NGN2 action potential firing from DIV7 to 35. Left panel: time course of spike rate for both genotypes. Right panel: significantly reduced spike rate on DIV28 in STXBP1 KO NGN2 neurons vs. ISO control. N = 20. (F) MEA recording of STXBP1 ISO control and KO NGN2 network bursting from DIV7 to 35-. Left panel: time course network bursts for both genotypes. Right panel: lack of network bursts on DIV28 in STXBP1 KO NGN2 neurons vs. ISO control. N = 20.
