Fig. 4
From: Detecting MUNC18-1 related presynaptic dysfunction and rescue in human iPSC-derived neurons
Calcium imaging demonstrating synaptic phenotype of STXBP1 KO NGN2 neurons. (A) Survival deficit in STXBP1 KO neurons quantified with CellTiter Glo. (B) Measurement of active cell counts in NGN2 neurons and NGN2 neurons co-cultured with human primary astrocytes for both genotypes. Left panel: time course quantifying active cell count from from DIV18 to DIV42. Right panel: active cell counts on DIV28. N = 10. (C) Measurement of coordinated network activity in NGN2 monoculture and co-culture with human primary astrocytes for both genotypes. Left panel: time course from DIV18 to DIV42. Right panel: quantifying coordinated network bursting on DIV28. N = 10. (D) Representative images from Incucyte Neuronal activity assay. Fluorescent images displaying the range in fluorescence for each pixel. *p < 0.1, **p < 0.01, ****p < 0.0001, two-way ANOVA. ****p < 0.0001, Student’s t-test. Error bars represent mean ± SEM.
