Fig. 5
From: Detecting MUNC18-1 related presynaptic dysfunction and rescue in human iPSC-derived neurons
Patch clamp recording of STXBP1 KO NGN2 neurons following STXBP1 re-constitution. (A) Illustration of AAV.PHPB capsid with STXBP1 construct. (B) Whole-cell voltage clamp recording of mEPSCs in STXBP1 ISO vs. KO neurons with control AAV and STXBP1-expressing AAV treatments. Left: quantifying mEPSC frequency. Right: quantifying mEPSC amplitude. (C) Example patch recording traces of mEPSCs. (D) Frequency distribution of mEPSC frequency. (E) Quantifying percentage of patched neurons with mEPSC present.
