Fig. 6

MEA characterization of STXBP1 KO NGN2 neurons following STXBP1 re-constitution. (A) Longitudinal MEA recording for mean firing rate demonstrating STXBP1 KO phenotype and dose-dependent rescue. Left panel: time course from 1 to 6 weeks. Right panel: mean firing rates on DIV35 are comparable across treatment groups. (B) Longitudinal MEA recording for number of network bursts demonstrating STXBP1 KO phenotype and dose-dependent rescue. Left panel: time course from 1 to 6 weeks. Right panel: number of network bursts, burst magnitude, and burst fraction on DIV35 across treatment groups. N = 20. ****p < 0.0001, one-way ANOVA. (C) Histogram of well spike counts over 20min recording demonstrating dose-dependent rescue of network bursts. (D) Relative STXBP1 protein expression in STXBP1 KO NGN2s following AAV transduction to WT NGN2 neurons at DIV21, 28, and 35. (E) Correlation between MEA activity parameters relative to STXBP1 protein expression on DIV35. *p < 0.1; ****p < 0.0001; ns not significant; one-way ANOVA. Error bars represent mean ± SEM.