Fig. 7 | Scientific Reports

Fig. 7

From: IgM+IgD− B cells in human gut-associated lymphoid tissue have memory features and give rise to IgM+ and IgA+ antibody-secreting cells

Fig. 7

12-hr-old repertoire analysis. (A) CD19+ B cell populations in normal appendix tissue of 12-hr old newborn compared to appendix from a 65 YO adult. (B) Metadata table for each B cell subset acquired from the 12-hr old donor. (C) CDR3 length distribution of 12-hr old donor stratified by subset. The clones in IgM + IgD + cells have longer CDR3 lengths than in IgM + IgD- cells (one-sided Mann-Whitney, p < 0.0001). Further, IgM + IgD-CD27- clones have longer CDR3s than IgM + IgD-CD27 + clones (one-sided Mann-Whiteney, p < 0.0001). (D) VH gene usage stratified by subset. Each clone counts only once. (E) Sample lineage trees of large clones with IgM+ IgD− sequences. All three clones include the germline VH gene sequence (large node at the top of the tree with 0 mutations). Nodes are colored by subset using the same color scheme as in panel C. Gating strategy for cell sorting (Supplementary Fig. 5).

Back to article page